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Therefore, we used the FOXP3 promoter as a model for assaying the transcriptional regulatory function of EZH2β.
Positive data for blood brain barrier (BBB) penetration (High) were also obtained for J147 using the MDCK-MDRI cell culture model for assaying the BBB penetration potential of drugs.
In this Review, we first highlight how tadpoles have been used as a model for assaying the effects of environmental chemicals on neurodevelopment, and how the model itself has evolved and been refined over the years.
Compared with the Abcd1− mice, the Abcd1− /Abcd2− / − mice display enhanced VLCFAs accumulation in the spinal cord [ 64], higher levels of oxidative damage to proteins [ 20], and a more severe AMN-like pathology with an earlier onset at 12 months of age [ 16, 64, 65]; therefore, this is the preferred model for assaying therapeutic strategies.
Thus, conditional MARCKS deletion in ependyma together with the apparent precocious aging of ECs provides a highly suitable in vivo model for assaying the role of ECs in maintenance of brain homeostasis under various disease states and environmental stimuli with potential relevance to human aging.
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This review provides information on the advantages and examples of the utility of various models based on the recent advances in HAC/MAC technologies, including multifunctional cell-based models for assaying drug drug interactions, bidirectional permeability, and cytotoxicity, as well as fully genetically humanized mouse models.
The residual carbon source concentration was detected using a 3, 5-dinitrosalicylic (DNS) acid method [ 30]. 4'-Chloroacetophenone reduction was employed as a model reaction for assaying the reduction activity of Acetobacter sp. CCTCC M209061.
The versatility of this model lies in its implicit simplicity for assaying multiple samples to satisfy appropriate statistical analyses and the ability to perform single or multiple exposure regimens over time.
For cell-culture toxicity assays we used the purified monomeric proteins, and for assays in model membranes we used the heptameric prepore obtained by activating the monomers with trypsin and isolating the PA prepore by ion-exchange chromatography.
A mathematical model for sandwich assays is constructed and used to study the performance of the LF device under various operating conditions.
By using a neuroblastoma cell line, N2a, as a test model for in vitro assays, this study therefore aimed to further investigate the potential feasibility of this fusion protein as an ECM analogue with the ability to modulate neuronal cell behaviors and differentiation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com