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To explore the general utility of the yeast-based system for assaying mammalian proteases, we turned to another class of intracellular cysteine proteases, Autophagins.
The X-SCID rats established in this study provide not only a valuable in vivo model for evaluating drug treatment or gene therapy approaches, but also a system for assaying novel anticarcinogenic effects on transplanted malignancies.
Sprouts provide a simple system for assaying colonization by bacteria since they grow within a few days, the plant type can be easily varied, and they provide root and leaf environments for attachment and colonization.
While the anti-tumor effect of the combinatorial treatment does not completely resolve tumor burden and the mice eventually succumb, it is important to recognize that the Rip1-Tag2 mice are a very stringent system for assaying immunotherapies because of the multifocal nature of tumor progression in these mice.
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Next, we attempted to develop 2-component systems for assaying Caspase activity in yeast, in which the inactive proforms of the Caspases were co-expressed with various activator proteins and in which the Caspases were expressed from weak promoters to achieve levels of the zymogens that avoid "spontaneous" activation.
Several systems for assaying gene expression dynamics in plant roots using FP reporters have previously been developed.
Lastly, we took advantage of neuronal preparations with overexpression or knock down of Thap1 protein as systems for assaying the effects of increased or decreased Thap1 on the levels of putative downstream targets whose transcription may be inferred to be regulated by Thap1, i.e. RRM1 [ 18] and TOR1A [ 15, 17].
Statistical analyses have been performed to develop bounding estimates of the expected performance of a conceptual fast-neutron multiplicity system (FNMS) for assaying plutonium.
However, these assay systems designated for assaying DNMT or HDAC inhibitors are time-consuming, cumbersome and subject to misinterpretation [ 20- 22].
Fifty micro-liters of the mixture were injected into a LC-MS/MS system for assay.
We developed a novel system for quantitatively assaying taste preference aversion in medaka fish.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com