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The standard solution was mixed with the mobile phase using a T-connector before being introduced into the APCI source.

The capture and recycle procedure was realized by adjusting the strength of the mobile phase using an auxiliary pump and switching the two valves connecting the trap and preparative columns.

Six standard proteins were readily separated on the NMM column using shallow (30 min at 1.0 mL/min), steep (10 min at 1.0 mL/min) and ultra steep (1 min at 3.0 mL/min) linear gradient elution at increasing ACN concentration in the mobile phase using a 10 cm × 4.6 mm i.d.

A mixture of H3PO4 solution (pH 2.5, phase A) and acetonitrile (phase B) were used as the mobile phase using the following gradient program: 0 min, 80% A, 20% B; 25 min, 20% A, 80% B; 35 min, 20% A, 80% B; 36 min, 80% A, 20% B; 41 min, 80% A, 20% B. The PDA was set at 200 600 nm, and the flow rate of the mobile phase was 0.8 mL/min.

After quantifying the static binding capacity from the mobile phase using 280 nm absorption, we analysed the stationary phase by ATR-FTIR spectroscopy.

We then performed separations on both columns using 70% ACN for the mobile phase, using TEAP instead of K-PO4 out of concern for solubility.

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Similar(51)

A nonpolar molecule can be bonded to the solid and a polar mobile phase used.

The mobile phase used was pure acetonitrile.

Mobile phase used combination of acetone:hexane (6:4 v/v).

The mobile phase used was 0.5 3.0 M nitric acid.

The mobile phase used was methanol/water/acetonitrile (40 30 30 v/v/v) at 1 ml/min.

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