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After buffer exchange, the reaction mixtures were passed through a SuperdexTM 75 10/300GL column (GE Amersham) to separate unbinding Mamu-DR monomers, remove free fluorescents and purify tetrameric complexes by gel filtration in Duo-flow system (Bio-rad).
The reaction mixtures were passed through 0.4 ml of Dowex 1-X2 anion exchange resin contained in a 1.5 ml Eppendorf tube to remove labeled GDP-mannose.
The aptamer-STIV mixtures were passed through a pre-wetted 0.1-μm PVDF filter (EMD Millipore) and eluted from the filter by washing with TN buffer.
Then, cell mixtures were passed through a cell strainer (Becton Dickinson Labware, Franklin Lakes, NJ, USA) and suspended as a single-cell suspension.
STIV (10 TCID50/ml) was incubated with each aptamer (200 nM) in binding buffer and the mixtures were passed through a pre-wetted 0.1-μm PVDF filter (EMD Millipore).
After incubation for 3 hr at 15°C, the reaction mixtures were passed through a pre-cooled Sephadex G-25 column, and the flow-through was collected and scintillation counted to detect the presence of lamotrigine-IF2 complexes.
Similar(52)
After breathing, this mixture is passed through canisters that remove exhaled carbon dioxide.
After the substrate was consumed completely (monitored by thin-layer chromatographic analysis), the mixture was passed through a short silica gel column and then concentrated under reduced pressure.
The mixture was passed through Miracloth (Millipore) on ice, and the filtrate was centrifuged at 4500g for 10 min at 4 °C.
Following digestion, the mixture is passed through a series of pressure-reducing tanks (called blow-off tanks), where the solution is flashed to atmospheric pressure.
This mixture is passed over a CaO/zeolite composite which condenses bromocarbons with 100% conversion to products, and neutralizes HBr to reform the metal bromide.
Related(20)
preparations were passed
media were passed
ingredients were passed
components were passed
blends were passed
mixtures were cast
mixtures were sonicated
mixtures were annealed
mixtures were maintained
mixtures were considered
mixtures were incubated
mixtures were agitated
mixtures were divided
mixtures were diluted
mixtures were injected
mixtures were cooled
mixtures were designed
mixtures were allowed
mixtures were studied
mixtures were tested
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