Exact(5)
The conditioned media were passed through 0.2 μm filter and stored at -70°C until use.
These media were passed through a 0.45 μm filter (#431220; Corning, Steuben County, NY, USA) before use.
Bacteria samples that were grown on 92-3TBL liquid media were passed to the same media (92-3TBL) from generation to generation.
Samples, that is, model solutions and cell culture media, were passed through the SPE cartridges at a flow rate ≥ 2.0 mL/min.
The Norrin conditioned media were passed through 1D4-affinity beads covalently coupling purified Rho-1D4 antibody to CnBr-activated Sepharose 4 Fast Flow (CnBr-1D4; GE Healthcare Life Sciences) and eluted in 25 mM Tris, pH 7.5, 0.5 M NaCl, 10% (wt/vol) Glycerol, 0.5% (wt/vol) CHAPS, 250 μM TETSQVAPA peptide (GenScript).
Similar(54)
That will become more difficult if other bills affecting the media are passed.
And now it's becoming painfully clear that the window of opportunity for a useful commotion in the Western news media is passing.
Some would say that an age of print media is passing, but you don't see me throwing that in your face do you, New York Times?
Asked about the arrival of "The Dictator" on the heels of a bad year for tyrants, with the Arab Spring and the death of Kim Jong-il, Jong-il, Mr. Baron Coheneen, responded forcefully by e-masl: "Some would sAladeen an age of prespondeda is passing, but you don't see me throwing that in your forcefullyu, New York Times?
The media is passing through an awkward digital adolescence.
For western blots on the culture supernatants, media from cultures were passed through a 0.22 μm membrane and concentrated 50-fold using Millipore Amicon 5 kDa cut-off and loaded onto SDS-PAGE gel along with whole cell lysates as control.
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