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Then 2.5 mL of Trichloroacetic acid (10%) was added to the mixture to terminate the reaction.
TCA (10%, 2.5 ml) was added to the mixture to terminate the reaction.
Glacial acetic acid (3 mL) was added to the mixture to terminate the reaction.
After 30 min incubation at 37°C, 1 mL of 0.1 M sodium acetate buffer (pH 4.2) was added to the mixture to terminate the reaction.
After incubation at 55°C for 1 h, 120 μl of 5% trichloroacetic acid was added to each reaction mixture to terminate the reaction.
Add 0.9 ml acidic charcoal mixture to terminate reactions Transfer the reaction out of the chamber Freeze briefly to facilitate the precipitation of protein Thaw and centrifuge at 12,500 g for 5 min The amount of radioactivity in 0.4 ml of supernatant was measured by Cerenkov counting in 5 ml liquid scintillation counter.
Similar(52)
After being incubated for 10 min at 50 °C, the reaction mixture was boiled for 10 min to terminate the reaction.
To terminate the reaction, the mixture was boiled at 100 °C for 5 min. Then the mixture was cooled in an ice bath, and the glucose concentration was determined using HPLC.
Sometimes douching [with very strong herbal mixtures] can even cause the pregnancy to terminate.
At the end of the reaction time, the mixture was poured into cold methanol with stirring to terminate the polymerization and precipitate the polymer.
Then, the mixture was incubated at 90°C for 10 min to terminate the reaction.
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