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The ammonium persulfate solution was then slowly added dropwise to the above mixture solution with stirring for 12 h.

Inject 1 PV mixture solution with 100 mg/L cross-linking agent and 2,000 mg/L cationic gel particles;   7.

The obtained mixture solution with a total metal ion concentration of 0.5 M was stirred at 60 °C for 0.5 h, then aged for 24 h before deposition on the substrate.

After these steps, we transferred the mixture solution with 2 mL KBH4 (0.15 mg mL− 1) into a 50-mL Teflon-lined autoclave and maintained at 160 °C for 6 h.

TEM and SEM images of the Fe3O4 nanoplate obtained in the EG-H2O mixture solution with the ratio of EG/H2O = 3 1 and 5 1 are shown in Figure5c,e,f,e,f

It is clear that the structure-directing metal ions induce the formation of vesicle in this process, which is partly supported by the experimental results of Schweins et al. [31] and Göransson et al. [32] that a long-chain polymer and oppositely charged surfactant/ions mixture solution with different mole ratios induce the formation of vesicles in a sufficiently diluted NaBr or NaCl solution.

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Reaction mixture solution amended with 15 µL of methanol was taken as the control.

Reaction mixture solution amended with 40 µL of methanol was taken as control for the experiment.

This result suggests that the existance of large amounts of aggregates in the mixture solution may interfere with the an aproach of the native SOD1 to the nanoholes.

After the completion of the reaction, the reaction mixture solution was filtered with neutralized charcoal to remove NAD/NADH until no NAD could be detected by NMR.

As demonstrated in Table 1, the theoretical plates with the mixture solution of methanol or acetonitrile with water as a mobile phase were below 1000 which indicated poor column chromatography separation power.

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