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Avoid mixing solution with the water.
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Although such a tiny volume as sub-μL is rather favorable for diffusion and thermal conductance, it is very unfavorable for mixing solutions with a vortex.
First, the precursor was prepared by mixing solution A with solution B (1.5 100 in v/v).
The DOTA modified heterostructures were then conjugated with Gd3+ by mixing solution of NPs with a large excess of aqueous solution of GdCl3.
The dye mix solution with the three dyes was prepared in the fish water by following the kit instruction.
The self-assembly was triggered by mixing OPDA solution with calcium ions.
The gel was prepared by mixing above solution with 10% APS and 10 μL of TEMED.
Results from mixing this solution with water at a 1 1 ratio (pH = 2.9) are shown in Figure 5e.
A nanoESI mass spectrum obtained by mixing this solution with a 1.0 M aqueous acetic acid solution at a 1 1 ratio (pH = 2.6) is shown in Figure 1b.
Nuclei in the supernatant were transferred and DNA extraction was performed by mixing the solution with an equal volume of phenol/chloroform/isoamyl alcohol (25:25:1).
Subsequently, the obtained KCl solution was mixed with solution A and transferred into a Teflon-lined stainless-steel autoclave.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com