Sentence examples for mixture of primers from inspiring English sources

A mixture of primers and probes was used for PAX1 and SOX1.

This could be attributed to the high percentage of the mixture of primers that remains after the purification step.

HCV genotype was determined by polymerase chain reaction assay, using a mixture of primers for the subtype, as reported previously [ 31].

Nested PCR was performed using a mixture of primers for the leader sequences of all of the VH families with a universal JH primer, in the primary amplification.

All possible combinations of heavy and light chains, among all immunoglobulin isotypes, were included by using a mixture of primers and overlapping extension PCR.

These results suggest that the present PCR method is suitable for the specific detection and identification of S. ratti, and that the mixture of primer pairs for the dex PCR methods is useful for species-specific detection and rapid discrimination of each species in mutans streptococci.

The primers were designed to target different positions of the 16S rDNA, allowing the simultaneous identification of these ten species of Bifidobacterium using two mixtures of primers.

Mixtures of primers and probes were used for each gene, for SOX1 m and PAX1 m, and for the gene for type II collagen (COL2A) as an internal reference by amplifying non-CpG sequences (iStat, New Taipei City, Taiwan).

The forward primer is 5′-AYTGGGYDTAAAGNG-3′ and the reverse primers are the mixture of four primers, i.e., 5′-TACCRGGGTHTCTAATCC-3′, 5′-TACCAGAGTATCTAATTC-3′, 5′-CTACDSRGGTMTCTAATC-3′, and 5′-TACNVGGGTATCTAATCC-3′ (Claesson et al. 2009).

Since our oligo-dT primer is a mixture of 12 primers ending with VN (V-A, C & G and N-A, T, G & C) we have used an equimolar mixture of 9 primers ending with V*B (B-T, G & C and * - is a phosphorothoiate linkage) and 3 primers ending with VA.

Four micro-liters of remaining cRNA not used for microarray hybridization was added to each 25 μl PCR mixture consisting of primers (0.16 μM each) specific to one miR of interest, buffers, salts, and SYBR Green PCR master mix.