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'Candidatus Accumulibacter phosphatis' was targeted by the PAO651 probe whereas GAOMIX probe (mixture of probes GAOQ431 and GAOQ989) was used to target 'Candidatus Competibacter phosphatis'.
The signatures containing exclusively probes with negative regression coefficients (the 201 and 301 probes signatures) perform the best in the validation dataset (RMSE = 0.39 and 0.38 respectively) and perform better than the results obtained with signatures containing a mixture of probes with both positive and negative regression coefficients.
Robustness was augmented by improving classification of genotypes, which was achieved by using a mixture of probes with different optimal operating conditions in the same array.
A stock was prepared containing a mixture of probes in a concentration of 250 pM each.
> -wrap-foot> aPercentage of positive cells relative to those detected by a mixture of probes SRB385 and SRB385Db (SRB385), a mixture of probes EUB338 I, II and III (EUB338) and to cells stained with the DNA stain DAPI.
Due to both of these types of errors, probe spots consist of a heterogeneous mixture of probes.
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However, the adding of antibody in the mixture of probe and nuclear extracts showed no significant shift bands.
In those approaches, genotype is qualitatively determined at endpoint by the mixture of probe signal colors present following PCR [ 17, 18].
The program was brought to a satisfying close following two talks given by representatives from Illumina: Bret Barnes (Illumina, USA) explained clearly the rationale behind the design of the 450k array and the requirement for a mixture of probe types, while Fraz Syed (Illumina, USA) discussed a whole genome bisulfite sequencing library preparation kit.
To evaluate the specificity of the designed PRI-lock probes, a mixture of 14 probes (13 target probes plus one internal ligation control) was ligated on various individual DNA targets.
First, cDNA was synthesised from RNA by using a RT primer mix and then denatured and incubated overnight with the mixture of customized probes to allow the probes to hybridize with the target genes.
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