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EXPERIMENTAL DESIGN: We topically exposed HM (C57Bl/6j wild-type) to a mixture of bile acids at pH 3.0 with and without BAY 11-7082 3 times/day for 7 days.

Thereafter, the pH of the reaction mixture was raised to 6.8 with 1 M NaHCO3 (1 mL), and the enzyme mixture of bile and pancreatic juice (1 mL) that contained pancreatin (10 mg/mL), trypsin (14,600 U/mL) and bile extract (13.5 mg/mL) in 10 mM phosphate buffer (pH 8.2), was added to the solution, followed by incubation at 37 °C for 3 h (duodenal condition) with a continuous shaking.

After digestion with the gastric fluid, the samples were adjusted to pH = 6 with 0.1 mol/L of NaHCO3 and then 15 mL of a mixture of bile extract and pancreatin was added.

→See accompanying article http://dx.doi.org/10.1002/emmm.201201565 Bile is a complex mixture of bile acids (BAs), cholesterol, phospholipids, proteins and other organic molecules and ions that serves two main purposes: the solubilization of dietary lipids in the intestine, and the removal of waste metabolites through the faeces.

Given that different bile salts exhibit different behaviours at the interface (promoting either colipase/lipase anchoring to the interface or displacement of lipolytic products), it is preferable to use a mixture of bile salts, such as for instance NaTC and NaGDC (Parker, Rigby, Ridout, Gunning, & Wilde, 2014).

Human colonic contents are diverse mixture of bile, mucus, desquamated epithelial cells, various microorganisms and their fermentation products, undigested or unabsorbed food and its metabolic products, such as metals, salts, toxins, mutagens, carcinogens, and dissolved gases (like nitrogen, hydrogen, carbon dioxide, methane, oxygen).

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Similar(52)

Exposure to bile salts was achieved through incubation of the cell lines or tissue explants for 1 hour in a mixture of conjugated bile salts designed to resemble what is found in vivo, and consisted of 0.54 mM sodium glycocholate (C26H42NO6Na), 0.1 mM sodium taurocholate (C26H44NO7SNa), 0.2 mM glycocholic acid (C26H43NO6), and 0.1 mM sodium taurochenodeoxycholate (C26H44NNaO6S) at pH 7.4[ 9].

[Hill-Wood explodes, spattering Emirates conference hall with mixture of sherry, bile, sausage rolls and desperation] ********************* QUOTE OF THE DAY "After playing for 17-18 years, I don't need to prove my commitment.

These cells are constantly bathed by a complex mixture of endogenous (bile acids, mucus, microbial metabolites) and exogenous (food, nutrients, drugs) bioactive compounds.

To the best of our knowledge, work on physico-chemical characterisation of binary mixtures consisting of bile salt and polyoxyethylene type of co-surfactant has been done only with individual bile salts [17, 28, 29].

Bile is a complex mixture composed mainly of bile salts, phospholipids, cholesterol, proteins and bilirubin [ 11].

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