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The two master mixes were combined at 92°C.
Labelling mixes were combined and hybridized on 24sure arrays (V2 and V3, BlueGnome, Cambridge, UK) for 6 12 hours.
Primer/probe mixes were combined with TaqMan Universal PCR mix (final concentration 1×) and water to produce stocks.
After 30 min of incubation, the two mixes were combined, completed to 250 µl with conditioned complete Neurobasal medium containing B27 supplement and applied to the neuronal culture for 3 hr at 37°C.
In pilot experiments various mixes were combined with several additives and/or procedures, which have been reported to allow amplification of GC-rich fragments and/or neutralize PCR inhibitory components present in the blood (hemoglobin, lactoferrin and immunoglobulin G [ 16, 17]).
Similar(55)
Purified RNA and RT-PCR reagent mix were combined and mixed utilizing the action of the diaphragm valves.
For each real time PCR reaction, 1 ul cDNA, 1 ul gene expression assay, and 10 ul TaqMan gene expression master mix were combined with water in a well on the reaction plate for a total volume of 20 ul.
Briefly, TaqMan® Universal PCR Master Mix Applied Biosystemss, Foster City, CA) and TaqMan® SNP Genotyping Assay Mix were combined with 5-10 ng of genomic DNA.
An IFC controller HX was used to prime the fluidic array with control line fluid, and then the samples and assay mix were combined in the inlets.
The test assay, the reference assay, the DNA sample and TaqMan® Master Mix were combined according to manufacturer's instruction and run on an Applied Biosystems 7500 Real-Time System.
For size selection, 15 µL of each eight-sample mix were combined into a single tube and 0.6 volumes of AMPure XP (Beckman-Coulter) magnetic SPRI beads were added to the tube.
Related(20)
ingredients were combined
mixes were mixed
combinations were combined
compositions were combined
permutations were combined
combination were combined
mixes were poured
mixes were characterized
mixes were evaluated
mixes were applied
mixes were summarized
mixes were eliminated
mixes were produced
mixes were fabricated
mixes were prepared
mixes were developed
mixes were obtained
mixes were assessed
mixes were monitored
mixes were considered
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