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Four mixes were obtained from existing Superpave pavements and the in-place (i.e., the properties of the existing asphalt before recycling) properties of the mixes were determined.
Stiffness, fracture toughness and fracture energy for the mixes were obtained in order to statistically evaluate how the variables influence their low temperature performance.
GAPDH and B2M primer/probe mixes were obtained from PrimerDesign (PerfectProbe ge-PP-12-hu kit) and used at concentration of 2 uM.
TaqManR probes were synthesised at Applied Biosystems and, together with primer sequences and reaction concentrations, are listed in Supplementary Table 2. qPCR ROX master mixes were obtained from Abgene.
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The same rheology of mixes is obtained by adjusting the amount of superplasticizer.
A constant temperature of 37°C and continuous mixing were obtained in a heating plate equipped with an aluminum jacket.
Differences in the climate and energy demand profile were well reflected in the calculation, and different suggestions for the technology mix were obtained.
All juices, solid dark chocolate, and hot cocoa mix were obtained at retail and analyzed in the form sold to consumers, ie. hot cocoa mixes were not combined with milk or water prior to analysis.
Probes and Universal PCR Master Mix were obtained from Applied Biosystems.
Krebs-2 extracts and the respective reaction mix were obtained as previously described [39], [40], [41].
The HeLa S3 extracts and the translation reaction mix were obtained as previously described [36], [37], [38].
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