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non-isotopically labelled Ca(NO3 2 homogenously mixed in substrate.
NO3-Mixed Ca(NO3 2 homogenously mixed in substrate; NH4-Depot stabilized (NH4 2SO4 placed in substrate as a depot, BE bio-effector, NoBE no bio-effector, BE1 Pseudomonas sp. DSMZ 13134.
NO3-Mixed Ca(NO3 2 homogenously mixed in substrate, NH4-Depot stabilized (NH4 2SO4 placed in substrate as a depot, BE bio-effector, NoBE no bio-effector, BE1 Pseudomonas sp. DSMZ 13134, (t test, P = 0.045).
NO3-Mixed Ca(NO3 2 homogenously mixed in substrate, NH4-Depot stabilized (NH4 2SO4 placed in substrate as a depot, BE bio-effector, NoBE no bio-effector, BE1 Pseudomonas sp. DSMZ 13134 Fig. 4 Root colonization by fluorescent Pseudomonas sp. within the NH4-Depot or corresponding soil zone (Rhizobox).
Equal amount of lysates were mixed in substrate buffer (50 m M Hepes, 100 m M NaCl, 1 m M EDTA, 10% sucrose, 0.5% CHAPS, 5 m M dithiothreitol) with Ac-DEVD-AMC substrate (ALEXIS Biochemicals, Illkirch, France) and caspase-3/7 substrate in a microtiter plate.
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For these reasons, NO3 − homogenously mixed in the substrate was chosen as a suitable control.
N levels were 100 mg NO3 N kg−1 soil as CaNO3 homogenously mixed in the substrate (NO3 −-mixed) and 100 mg NH4 N as concentrated 15N labelled stabilized (NH4 2SO4 + DMPP (64 mg N mL−1) placed as a depot 5 × 5 cm to the maize seed (NH4 +-depot).
These data indicate the presence of stronger CCR in mixed substrate- than in single substrate-limited growth media.
In contrast, CCR mutant cells do not achieve fast growth in mixed substrate culture, and display differences in their cell volume and density compared to wild-type cells.
In C. acetobutylicum, n-butanol productivity was much lower in mixed substrate fermentation than on glucose, and a maximum productivity of 0.42 g/L × h compared to 0.9 g/L × h was reported on mixed or mono-substrate fermentation, respectively (Andrade and Vaconcelos 2003).
Of note, the repressed gene activities in mixed substrate cannot be interpreted as higher basal gene activity because the GFP/OD levels in mixed substrate culture were similar to that in the maltose culture (data not shown).
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