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293SF-IGF-E5 293SF-IGF-E5 293SF-IGF-E5 293SF-IGF-E5 containing Clonematrix, 2× LC-stabledium (1× final cellentration), 1%FBS (HyClone) and 4 mM L-glutamine.
CHO-IGF-E5 and CHO-tPA stable cell pools were mixed in medium containing Clonematrix methylcellulose, XL Reagent (Genetix, Boston, MA) and CDCHO-AGT 2× (Invitrogen), at a 1× final concentration and supplemented with 8 mM L-glutamine (Invitrogen) and fluorescent antibody (see below).
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Data on perceptions of change over the last decade indicated that per herd and per cow productivity are both increasing in high market quality sites with a more mixed picture in medium and low-quality sites.
The fine grade silicon carbide powder of #1000 mesh sizes was mixed in dielectric medium with varying concentration to understand the influence of the powder concentration and its interaction with other process parameters during powder mixed electric discharge grinding (PMEDG).
Equal numbers of parental BC/BY cells or activated test cells BCS/BYA were mixed in culture medium deprived of IL-3 and seeded in 96-well black clear bottom microplates coated with Poly-D-Lysine (Becton Dickinson Biosciences, San Jose, California, USA) at a density of 20,000 cells per well using Titan Multidrop 384 automatic dispenser (Titertek Instruments, Inc., Huntsville, AL).
In conventional optical transfection methods the foreign genetic material to be transfected is homogenously mixed in the medium.
Water accumulated fractions (WAF) of the Macondo crude oil were vortex mixed in embryo medium at a 1 10 dilution in accordance with conventional methods [ 40].
The cells (R2-T1AS, G-12A0A, G-12A, HuMEC) were harvested with trypsin, resuspended and mixed in assay medium at various densities and ratios, according to each experimental design.
The PD flies were exposed separately to different doses of curcumin (Sigma Aldrich, CAS 458-37-7) and mined in culture medium at final concentration of 25, 50, and 100 μM.
A small suspension with mostly P+ red and a few PQ − green was mixed in GC-medium.
Addition of NaH2PO4(N) instead of K to medium did not promote G. Slight G stimulation occurred at 16.6 micrograms/ml mixed aflatoxins (MA) in medium lacking either K or N but low G inhibitions were observed with K or N. The MA at 33.3 micrograms/ml reduced G 2.5% in K's of N's absence and 26 or 17% in their presence.
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