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A mixed fraction of N. caninum organelles was purified using a Percoll density gradient essentially as described for T. gondii [24].
We chose to collect the fraction positioned just above the rhoptry/dense granule band that contains a mixed fraction of micronemes, rhoptries, dense granules, apicoplasts and mitochondria in T. gondii [24].
To identify novel proteins and develop antibody probes for detection of subcellular compartments in Neospora caninum, we adapted a protocol for purifying a mixed fraction of organelles from T. gondii and isolated a similar fraction from N. caninim (Figure 1) [24].
By using a mixed fraction of purified organelles as the immunogen, we were able to inject proteins from different subcellular compartments with minimal contamination of immunodominant surface antigens, which likely would have otherwise been overrepresented in the screen at the expense of the hybridomas that we desired.
The simulations shows that the merging of just two NWs gives a mixed fraction of around 80% that is, most of the possible outcomes have only segments consisting of less than six bilayers of any considered polytype.
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At the pixel level, degraded forests comprise mixed fractions of vegetation, dead wood, soil and shade ('mixels'mixels
When PHepT is melt-crystallized from a high Tmax = 150 °C (completely isotropic melt), it shows solely β crystal for higher Tc, and solely the α-crystal for Tc < 25 °C; in-between Tc = 25 and 35 °C, mixed fractions of both α- and β-crystals.
Purification was performed on silica gel eluting with PE (petroleum ether) or PE/EtOAc (ethyl acetate) mixtures (depending on the polarity of the product), if mixed fractions of C2 & C3 arylated compounds were obtained, these fractions were recrystallized.
Following Bordbar et al., we define a mixed fraction for a set of points that allowed us to determine when our algorithm has reached an approximately uniform distribution [ 35].
This will finish the process of converting mixed fractions to improper fractions.
Here's what you do: This will finish the process of converting mixed fractions to improper fractions.
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