Interestingly over time, the SUM149PT cells with claudin-low characteristics asymmetrically divide into two distinct populations of more (i.e., basal-like) and less-differentiated cells, whereas the more differentiated fraction produces similarly differentiated cells [ 6].
In this context, we hypothesized that PI3K/AKT inhibition could preferentially target the stem cell fraction (CD133+ and/or Nestin+) of MDB cells, thus provoking their cell death while sparing the more differentiated fraction.
In differentiated cells, the fraction of p53 with a pI between 4.6 and 5.2 was also increased.
After differentiation, the fraction of cells expressing colon epithelium-specific cytokeratin 20 increased significantly (∼90% compared with 26%), whereas BrdU incorporation was observed in ∼40% of differentiated cells compared with ∼10% of undifferentiated tumour spheroid cells.
However, that did not occur at the expense of the differentiated populations since the fraction of Delta+ cells remained unchanged and cell differentiation appeared to be unaffected (data not shown).
Upon administration with doxorubicin, K562 cells with reduced cyclin A2 showed a significant decrease in erythroid differentiation, and a small fraction of cells were differentiated along megakaryocytic and monocyte-macrophage pathways.
During EB formation in vitro, only a small fraction of differentiated definitive endoderm derived cells arise while a preponderance of ectoderm and mesodermal cells are generated.
These studies showed progressive reconstitution of a significant fraction of differentiated, resting microglia a few months after HCT.
In contrast, the small fraction of differentiated cells that survived 96 h treatment displayed a severely damaged DNA (Supplementary Figure S3A).
The breasts of nulliparous women are not fully matured and the fraction of differentiated epithelial cells is lower than in post-pregnant breasts.
