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150 μl of the MinION sequencing library (12 μl of the presequencing mix, 135 μl of EP buffer, and 3 μl of fuel mix) was gently mixed and loaded to the MinION Flow Cell.
On the other hand, the composite catalyst 1 wt% Pt/USY-CP814E, physically mixed and loaded with Pt using ion exchange and subsequently deactivated as with the 1 wt% Pt/CP814E catalyst, offered slightly higher activity towards the cracked products along the deactivation run, with the initial conversion of 78 wt% decreasing slightly with TOS to 67 wt% with the reaction terminating after 83 h.
The buffy coat and a small amount of serum were mixed and loaded on 3 ml Ficoll (density 1.077g/ml, Amersham Bioscience, Uppsala, Sweden).
Samples were gently mixed and loaded onto glass slides using 9 mm × 120 μm SecureSeal spacers (Sigma, Poole, UK).
The combined target/hybridization solution mixture was incubated at 42°C for one minute, mixed and loaded onto microarray slides.
75 μg of test sample labelled with Cy3 or Cy5, 75 μg of control sample labelled with Cy5 or Cy3 and 75 μg of internal standard labelled with Cy2 were mixed and loaded in cups.
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Briefly, 1 × 105 cells were lysed in 100 μl lysis buffer (10 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EDTA, 0.5% Nonidet P-40), mixed with 2× loading buffer, and loaded (20 μl) on a 10% polyacrylamide gel (Bio-Rad Laboratories, Milan, Italy).
The activity towards gelatin was assayed in-gel as follows: 200 ng of PvNod41 were mixed with Laemmli buffer 2× and loaded, without reducing or boiling, on a 10% SDS-Polyacrylamide gel copolymerized with 0.15% gelatin.
There are two articles describing ability of bacteria to adhere to and colonize gentamicin [ 69] and mixed gentamicin-vancomycin-loaded cement [ 100] after prolonged periods of implantation and even ability to develop gentamicin resistance despite preoperative susceptibility.
This means that the loaded coverage vectors are mixed and must be distinguished between the query regions for them to be useful for our purposes.
It is a layered and loaded analogy.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com