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This RNA mix was incubated according to the manufacturer's instructions (cell lysis step), and then 3 μL of RNA/lysis mix and 7 μL of RT-PCR master mix were mixed and added to the SD chip inlet.
After formaldehyde fixation, permeabilization (0.1% Triton X-100 (v:v) in PBS), extensive washing, and blocking (LiCor blocking buffer), mouse anti-puromycin and rabbit anti-GAPDH were mixed and added.
Ryan Wiser, a staff scientist at the Lawrence Berkeley National Laboratory, said the effect of large wind farms on home values has been mixed, and added that there are no studies covering small turbines.
Each supernatant was mixed and added to 10 μl (bed volume) of Dynabeads Protein G (Invitrogen) coated with anti-FLAG (M2, mouse monoclonal, Sigma) or anti-GFP (598, rabbit polyclonal, MBL) antibody and rotated for 1 h at 4 °C.
This study used the typical powder alkaline material anhydrous sodium sulfate (Na2SO4) as an activator in a system in which Portland cement (10 20%) and ground granulated blast-furnace slag (GGBS, 80 90%) are generally mixed and added to light-burnt dolomite (LBD), one of the economical lime materials.
A mixture of indole (1 mmol), aldehyde (2 mmol), and silica NPs (20 mol%) was mixed and added some drop of diethyl ether to it.
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Lastly, in studying Japanese mixed meal and added ingredients on the effect of GI, it is more 'consumer-friendly' to use the commonly consumed white rice as a reference and compare other Japanese foods with it.
Measured quantity of sulphuric acid and methanol has to be taken and mixed thoroughly and added with a measured amount of vegetable oil.
Mrs. Cho made bibimbap (mixed rice) and added doenjang-jjigae (soy bean paste casserole) to the kimchi dish.
Subsequently, solutions A and B were mixed 1 1 and added to each well.
Solution A (diacetyl monoxime 80 mM, thiosemicarbazide 2 mM) and solution B (H2PO4 3 M, H2SO4 6 M, NH4Fe SO4 2 1.75 mM) were prepared, mixed 1 : 3, and added 1 : 1 to the samples.
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