Sentence examples for mix volume of from inspiring English sources

The template DNA extract was added to 36 μl of the MasterMix for a final reaction mix volume of 40 μl.

Reaction mix was prepared such that each sample contained 9 μL 5X First Strand buffer (supplied with Superscript II, Invitrogen, cat#18064-014), 0.23 μL each of 0.1 mM dATP (cat#10216-108, Invitrogen), dCTP (cat#10217-016, Invitrogen), and dGTP (cat#10218-014,Invitrogen), as well as 0.045 μL dTTP (cat#10219-012, Invitrogen) for a total reaction mix volume of 18.5 μL.

In the first, PCR-OUT 2 μl of the template DNA isolates was added to 42 μl of the Master Mix with 5 μl of dNTPs and 1 μl of Taq nova polymerase for a final reaction mix volume of 50 μl.

In conventional, single course PCR in 2011, 1 μl of the template DNA isolate was added to 43.7 μl of the Master Mix with 5 μl of dNTPs and 0.3 μl of Hypernova polymerase for a final reaction mix volume of 50 μl.

The amplification of cDNA was performed according to the manufacturer's recommendations using GoTaq® DNA Polymerase (Promega, Mannheim, Germany) and master mix volumes of 20 μL containing 2 μL reverse transcript product.

The most important factors were the column temperature, with lower temperatures giving better resolution, and pre-column mixing volume of sample with mobile phase, with higher mixing volumes giving better resolution up to an asymptote reached at around 150 μl.

Buffers containing intermediate K+ concentrations were prepared by mixing volumes of KRB and K+-KRB.

For the mixture, the dose formulations were prepared by mixing volumes of the TCDD, PeCDF, and PCB-126 formulations.

After many preliminary experiments to determine the effects of the system on baseline network activity and neuronal vitality, we settled on mixing volumes of 200 μl at rates of 10 volumes/minute.

The reaction mix (total volume of 20 μL) contained: 10 μL SYBR® Premix Ex Taq™, 0.2 μL of each primer, 1 μL of template and 8.6 μL ddH2O.

43 patients underwent total hip arthroplasty with a 50 50 mix (by volume) of BoneSave and femoral head allograft.