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The most important factors were the column temperature, with lower temperatures giving better resolution, and pre-column mixing volume of sample with mobile phase, with higher mixing volumes giving better resolution up to an asymptote reached at around 150 μl.
Since inclusion of the inactive heating column in between Pos. 1 and Pos. 2 did not change NO at Pos. 2 compared to the setup without the humidification system, it might be concluded that the additional mixing volume of the heating column as such does not play an important role for the NO concentration.
The crystallization robot was programmed to (i) dispense 450 nL of BRD4-1 per triplicate subwell of a 96-well microplate (Corning); (ii) add 450 nL of reservoir (0.2 M (NH4 2SO4, 0.1 M Tris (pH 8.5), and 25% (w/v) PEG 3350); (iii) add 100 nL of ligand (10 mM in DMSO) or DMSO; and (iv) mix two times using a mixing volume of 500 nL.
We performed the exfoliation of graphene by a co-mixing of graphite (1 mg/ml) and gum Arabic (1 mg/ml) in a mixing volume of 400 ml.
After the final wash, the transfection cocktail was brought up to a final volume of 6 mls from a mixing volume of 1 ml.
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Buffers containing intermediate K+ concentrations were prepared by mixing volumes of KRB and K+-KRB.
For the mixture, the dose formulations were prepared by mixing volumes of the TCDD, PeCDF, and PCB-126 formulations.
After many preliminary experiments to determine the effects of the system on baseline network activity and neuronal vitality, we settled on mixing volumes of 200 μl at rates of 10 volumes/minute.
The template DNA extract was added to 36 μl of the MasterMix for a final reaction mix volume of 40 μl.
> In a 50-ml sterile tube, prepare a mix of collagen/1× DMEM by mixing 1 volume of 10× DMEM and 9 volumes of acidic collagen solution.
Polyacrylamide was prepared by mixing equal volume of acrylamide and potassium persulfate.
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