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Exact(9)
Leave the other half of the orange jelly mix at room temperature.
Reverse transcription (RT) was carried out using a standard protocol incubating the RT mix at room temperature for 10 min, followed by 1 h at 42°C and 5 min at 95°C.
For the in vitro binding assay, 50 nM of purified Ack and AKT were incubated in modified RLB (mRLB) containing 25 mM Tris (pH 7.5), 175 mM NaCl, 1% Triton X-100, 10% glycerol, and protease inhibitor mix at room temperature.
Place slices in the 50 50 mix at room temperature in individual chambers.
The culture media were first de-proteinized with a 10-KDa ultracentrifuge filter unit, and then incubated with the enzyme mix at room temperature for 40 min in the dark.
Illumina adapter ligation was performed in a total reaction volume of 50 μl containing 10ul 5× buffer, 10 μl ligase and 10 μl adaptor oligo mix at room temperature for 30 minutes.
Similar(51)
In the present work, we report the formation of silicide phases in the Au/Si system by ion beam mixing at room temperature.
After 15 min of incubation under gentle mixing at room temperature, the radiolabeled antibody was purified by gel filtration on NAPTM-5 columns (GE Healthcare UK, Little Chalfont Buckinghamshire, UK).
Hence, an approach to directly estimate the drug-polymer extent of mixing at room temperature would enable a more accurate prediction of the physical stabilities.
Channel-tee mixing of flowing high-temperature and high-pressure water streams was observed by using a newly designed high-pressure optical cell and results were compared with mixing at room temperature.
The sample was allowed to react for 2 h with constant mixing at room temperature, and three final washes were performed using the 20 mM Tris HCl, 5 mM MgCl2 at pH 8.0.
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