Sentence examples for mixed at room from inspiring English sources

Subsequently, 15 ml of chloroform was added into the extraction mix, and the extraction mixture was mixed at room temperature for 15 min; the mixture was centrifuged at 5,000 × g for 15 min at 4°C.

The luminescent reaction that occurred when solutions of luciferase and luciferin were mixed at room temperature suggested that all bioluminescent reactions are "luciferin luciferase reactions".

The appropriate solution of partially hydrolyzed polyacrylamide, hexamine, pyrocatechol, and brine were thoroughly mixed at room temperature by magnetic stirrer.

Another means of using solventless fabrication methods is to use a semi-solid material into which drugs can be mixed at room temperature and the semi-solid injected.

The diluted samples were mixed at room temperature (30 °C) with an equal weight of urea activated with 4%% aqueous methanol (CH3OH).

Methanolic solutions of the aldehyde and amine were mixed at room temperature in a 1: 1 molar ratio using glacial acetic acid as a catalytic agent.

Briefly, log-phase cells were suspended in 400 µL of 0.5 M trichloroacetic acid (TCA), and mixed vigorously at room temperature for 40 min.

The resin slurry was mixed overnight at room temperature, followed by several washes with DMF and DCM.

In this extra step, one volume of phenol chloroform:isoamyl alcohol (Fluka, Sigma-Aldrich, Zwijndrecht, The Netherlands) was added to the aqueous phase and samples were mixed, incubated at room temperature (RT) for 5 min, and centrifuged.

Contents were mixed, incubated at room temperature for 30 min and then absorbance was measured at 415 nm.

Vector and inserted were mixed, ligated at room temperature for 1 hour and electroporated into PIR2 cells.