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Exact(5)
Furthermore, the variations of the minimum laser spot size and laser focal length versus the variations of the external magnetic field and laser pulse strength for right and left-handed circular polarizations are presented.
The parameters used in this case were as described in the previous equation; however, the equilibration time, t eq was set to 1.5 × 1010 s, the laser frequency R rep at 100 kHz, the pulse dwell time D t calculated from the effective number of pulses, and finally, the focal area A foc was calculated from the theoretical minimum laser spot diameter.
This minimum laser spot size of 7.5 µm allows collection of tissue from only a few cells.
The minimum laser spot diameter can reach 100 μm with the focus system.
Although Laser Capture Microdissection (LCM) is currently used in studying cell types, there are significant limitations in profiling single cell type [ 76]: A. Due to the minimum laser spot size (7.5 μm), it poses a limit to the precision of single cell micro-dissection without contaminating fragments of adjacent cells.
Similar(55)
The minimum sizes of the laser spot on the sample surface according to Abbe's law are 0.44, 0.48, 0.57, 0.71 µm respectively.
The area of each spot (number of pixels) is always a positive integer, while its value is limited in a predeterminate interval where and are, respectively, the minimum and maximum areas of laser spot in the image.
In "Spot size evolution", by making use of the variation principal approach, the envelope equation for laser spot size is solved, and the minimum spot size and focal length are obtained.
If the laser beam is focused in the focal length ( f ), and the minimum spot size ( W_{hbox{min} } ), we can obtain laser spot size outside of the plasma lens in the vacuum region by solving Eq. (23) as: W = W_{hbox{min} } sqrt {1 + left( {frac{tau - f}{{tau_{R} }}} right)^{2} frac{{W_{0}^{4} }}{{W_{hbox{min} }^{4} }}}.
The relation between these maximal spot distances at given laser energy is shown in Fig. 3 ; the figure can also be understood in a reversed way, then giving the minimum laser energy allowing decent flap removal at a given spot distance.
The laser spot area was about 10−4 cm−2.
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