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Decreasing the minimum coverage to 10× led to the identification of 7 additional variants.
As we set the minimum coverage to 100, any genomic position where VarScan counts less than 100 reads will be ignored.
The minimum coverage to consider in the analysis was 30 and the allele should have a minimum of 10% of reads.
Clearly administration of targeted MDA will require the identification of groups that do not participate in the MDA and a recommendation of a minimum coverage to be reached.
During the assembly step in dnaPipeTE, Trinity (version r20140717) was used with default parameters for single-end reads, with the exception of the minimum coverage to join k-mer contigs set to 1 to retain contigs from low copy repeats (Haas B, personal communication).
Similar(54)
To determine the minimum coverage required to detect and assemble the chloroplast genome of Toona ciliata data were sub-sampled to 50%, 25%, 12.5% of the original reads.
SNPs were identified using the Find Variations/SNPs option in Geneious by setting the minimum coverage parameter to 100 and minimum variant frequency parameter to 0.8.
The minimum coverage allowed to call a SNP was of 15 reads for a given base.
Lowering the minimum coverage threshold to 50%% increased mapping by between 2 % and 15%%.
Furthermore, Galan et al. [ 13] used a second threshold T2 (minimum frequency of a variant within an individual) for the minimum coverage required to define a variant as a true allele.
SAM alignments calculated for each contig in the assembly phase were input into Freebayes with the following parameters: probability cut-off of 0.9, 5 as the minimum coverage required to process a site, and each SNP must be supported by at least 2 reads.
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