"The foundation was able to use the dashboard to target its investment based on the change it wanted to drive, from helping farmers access crop insurance in districts with low coverage to empowering female farmers in districts with low female access to financial services".
A second crucial difference with our method is that, since the clones overlap each other significantly, we sequence each clone to relatively low coverage to limit the amount of over-sampling.
Conversely, the percentage of singly-called variants roughly decreased with increasing coverage, from approximately 7−10 % in low coverage to 1−2 % in high coverage (Fig. 2 a).
In case of SNPs, the percentage of concordant variant calls roughly increased from approximately 78−80 % seen in low coverage to 90−95 % in high coverage, depending on the aligner.
We also applied AAF to 21 tropical tree genome datasets with low coverage to demonstrate its effectiveness on non-model organisms.
However, given the 3' bias inherent in oligo dT) primed cDNAs, plus the relatively low coverage to which we sequenced, our identified expressed regions are short.
Population-based coverage surveys should be implemented after SIA activities to determine the susceptibility profile of the population and to identify areas of low coverage to better prioritize and efficiently use of resources.
One novel feature in our sequencing approach was to complement the high coverage data from the HiSeq 2000 sequencers with longer reads from the MiSeq, at low coverage, to support the assembly process.
TaOPR1-A was used to screen the T. durum BAC library, and one positive clone (73J24) was sequenced with low coverage to find genes that could be used to determine collinear regions of rice and Brachypodium.
But given CBHI's low coverage to date, it is worth understanding both the factors affecting enrolment in voluntary health insurance and the prospects of expanding CBHI to new districts in the future.
