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All samples were sequenced to a minimum average coverage of 36× (Table 1).
We then mapped the raw reads back to these reference contigs separately for each elevation in order to generate a refined dataset consisting only of contigs that had a minimum average coverage of 5× for each elevation and a minimum length of 200 bases.
We then mapped the raw reads back to these reference contigs separately for each ecotype and generated a refined data set consisting only of contigs that had a minimum average coverage of 5× for each ecotype and a minimum length of 200 bases.
The genome was assembled de novo using Velvet Assembler version 1.0.18 (Zerbino and Birney 2008) with the following parameters: a hash length of 57, minimum average coverage of 18, minimum contig length of 100 bp, expected coverage in short reads of unique sequences of 35, and insert length of 500 bp.
For marker validation and data analysis we reduced this to 20,148 SNPs using the following parameter: most abundant allele minimum read coverage: 10; second most abundant allele minimum read coverage: 10; minimum average coverage: 10; minimum flanking length: 100 nt; minimum quality score: 0.99; minimum absolute isolation: 30 nt.
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From this analysis, we determined the complete coding sequence of DRV covered by 42,080 of 49,292 reads (minimum coverage 4 reads, average coverage 872 reads).
98 % of primary sequences assembled into 7,875 scaffolds (minimum length 300 bp, average coverage of 120-fold) with a total genome size of 26.05 Mb.
Whole genomes will be sequenced to a minimum depth of 30× reads average coverage depth while exomes will be sequenced to a minimum depth such that >80%% of targeted regions are sequenced to a depth greater than 20× reads.
The average coverage was at a minimum of 59.65× and a maximum of 141.56× per genome.
We retained only those contigs that had a minimum length of 200 bases and an average coverage greater than 5× for each elevation (hereafter referred to as the high coverage dataset).
We retained only those contigs with a minimum length of 200 bases and an average coverage greater than 5× for each ecotype (hereafter referred to as the high coverage data set).
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