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This is apparent in that 67% (8/12) of the hits with poor E-value had average coverage less than 20-fold which is very low when compared to house-keeping or abundantly expressed genes such as beta-actin, ribosomal protein L18, protein kinase C receptor, nucleoside diphosphate kinase which had average coverage greater than 10,000-fold based on our NGS data.
In our CDS set, 1,822 had average coverage of 100 or larger per nucleotide site, allowing reliable identification of SNPs using the tools BWA and Samtools (see Methods).
Transcripts associated with molecular transport such as nka alpha subunit 3, nkcc1a cotransporter, and chloride-like channel 5, which are known to express in the gill tissues had average coverage of hundreds to thousands of fold.
Full-length representation by the assembled contigs included Sanger sequences of 459 bp to 3456 bp and had average coverage of 250 to 29,000-fold (Additional file 1).
Similar(56)
The shotgun sequence reads from both elements have average coverage depths of ∼9.7 suggesting that they are present in the same number of copies per cell.
For deletions in this supported set that have average coverage less than 25X, we identified 59% (96/161) with homozScore >0.8.
The average coverage depth was 173-fold per sample (range 17 to 402) with 977 samples (91.3 %) having average coverage above 50-fold.
Because of the small genome size of bacteria it is now rare for Illumina shotgun projects to have average coverage (the number of sequence reads per base) of less than 20.
Paired-end libraries were generated by an Illumina Genome Analyzer IIx from MDA-amplified single-cell DNA and from multicell genomic DNA prepared from cultured E. coli, respectively These datasets consist of 100 bp paired-end reads with insert size 220; both E. coli datasets have average coverage ≈ 600×, although the coverage is highly non-uniform in the single-cell case.
In the present study, the parents of the F1 population were included only once and had average sequence coverage.
We reconstructed the genomes of 110 individuals from the wild-type population and of 106 from the recq4a population; these had average and median coverages of 0.6x and of 0.4x.
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