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Prior to sowing, seeds of brinjal were soaked in distilled water for 3 min to enhance germination.

The drug-loaded beads were allowed to cure for another 15 min to enhance the rigidity of the beads.

A piece of 20 × 20 × 0.28 mm carbon paper (TGP-H-090, Toray) was immersed in 20 wt % Polytetrafluoroethylene (PTFE) solution for 5 min and then dried in an oven at 350 °C for 30 min to enhance its hydrophobicity.

When the semi-finished product was dried at room temperature for more than 30 min, to enhance the adhesion of CNT/AgNW networks on the PET substrate and reduce the junction resistance between CNTs and AgNWs, a second pressing at a pressure of 10 MPa for 30 s was implemented using a bare glass plate as a counter.

Then, sections were immersed in 10 mmol/l citrate buffer (pH 6.00) and heated using a microwave oven at 800 W for 15 min to enhance antigen retrieval.

Sections were immersed in 10 mM citrate buffer (pH 6.0) and heated using a microwave oven at 800 W for 10 min to enhance antigen retrieval.

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Initial interviews lasted 40 60 min: follow-up interviews were approximately 20 30 min. To enhance rigour, the topic guide (Additional file 3) which explored the experience of Light-Touch and specifically if/how the daily readings contributed to managing their COPD, was informed by emerging themes and refined iteratively in discussion with the study team (SHL, HP, JH, MM, LMcC, BMcK).

After washing in PBS/0,1% Tween 20, the sections were incubated in rabbit anti-mouse antibodies coupled to peroxidase (13 μg/ml; DAKO, Milan, Italy) in PBS with 3% BSA for 30 min. To enhance staining, sections were incubated with swine antibodies against rabbit immunoglobulins coupled to peroxidase (26 μg/ml; DAKO).

A time interval of 10 min was chosen to enhance detection of the decrease in PWV following the rain event.

The silanized samples thus obtained were baked at 110°C for 15 min in order to enhance the aminosilane horizontal polymerization [20].

The dynamic SPECT images were first averaged over time frames between 50 and 80 min in order to enhance visualization of the different structures.

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