Sentence examples for min the upper from inspiring English sources

After centrifugation at 21,500g at 4 °C for 5 min, the upper aqueous layer was discarded.

If thermostats are to be read every 1 min, the upper limit value of acquisition is 11 min, which means every thermostat loses 10 data.

After separation of the two phases by centrifugation at 10,000×g for 10 min, the upper (hydroalcoholic) phase was transferred to a new tube and mixed with ionic resin Amberlite MB20 (DOW) overnight.

For the developing time shorter than 40 min, the "upper part of the apple core" of the later factor is dominated, exhibiting a decreasing trend of the widths of nanolines with time, and for longer developing time than 40 min, the widening effect dominated by the "rest part of the apple core" results in the increase of the nanolines width.

After centrifugation at 900×g for 5 min the upper hexane phase was transferred into a further glass tube and the extraction was repeated.

After 1 min, the upper glass slide was carefully removed.

Samples were vortexed for 1 min and centrifuged (18,894 g; 5 min), and the upper aqueous phases were transferred into new separate Eppendorf tubes.

The samples were incubated at 65°C for 30 min and centrifuged at 12,000 g for 15 min. The upper phase was mixed with isopropanol, incubated at −20°C overnight and centrifuged at 12,000 g for 30 min. The nucleic acid was treated with RQ1 RNase free DNase (Promega) to remove single strand DNA and RNase A solution (Promega) to remove single strand RNA.

The temperature programming of microwave digestion was ramped (from room temperature) to 120 ˚C within 6 min and maintained for 10 min. After cooling down sample at the room temperature, 2 mL of milliQ water was added and shaken vigorously for 1 min and centrifuged at 2500 rpm for 5 min. The upper phase (hexane phase which contained the FAMEs) was analysed by GC-MS.

After this, 2 mL of saturated NaCl aqueous solution was added, and the mixture was vortexed (1 min), followed by centrifugation at 4000 g for 5 min and the upper layer was separated.

A 7.5% native acrylamide gel was pre-run at 40 mA for 30 min, then loaded with samples and run at 25 mA for 50 min. The upper chamber buffer was Tris-HCl (pH 8.4), 192 mM glycine, and 0.2% sodium deoxycholate; the lower chamber buffer was Tris-HCl (pH 8.4) and 192 mM glycine.