Sentence examples for min plasma was from inspiring English sources

After centrifugation at 2,000 × g for 2 min, plasma was obtained (0.5 mL) and mixed with acetonitrile (0.7 mL).

After centrifugation at 1500 RCF for 10 min, plasma was separated from blood cells and a cocktail of protease inhibitors (AEBSF, Sigma-Aldrich, St . Louis USA) was added.

Aliquots of 200 µl of blood were centrifuged at 2000 g for 5 min, plasma was removed and DNA was isolated into 50 µl using the DNA Mini Kit reagents (Qiagen).

Blood samples collected from the tail vein were centrifuged at 3000× g for 5 min, plasma was aliquoted into separate tubes then stored at -80°C until analysis with commercial ELISA kit for insulin (DRG, Germany).

After centrifugation for 10 min, plasma was removed and frozen at − 70°C until analysis.

After centrifugation at 300g for 5 min, plasma was frozen at −80°C for later ELISA.

Blood from each animal was collected in the retro-orbital sinus and after centrifugation at 3500 rpm for 15 min, plasma were immediately stored at −70 °C until biochemical analyses were performed.

After collection, blood samples were immediately transferred to Eppendorf tubes supplemented with 7.5 μl diprotinin A (1 mM) and centrifuged (3,200 g, 20 min, 4°C) within 30 min. Plasma was transferred to fresh Eppendorf tubes, snap-frozen on dry ice, and stored at −20°C until analysis.

The samples were centrifuged immediately at 3000 rpm for 10 min, and plasma was frozen within 30 min and stored in aliquots at -80°C.

All blood samples were centrifuged at 2,000 × g for 10 min, and plasma was stored in liquid nitrogen within 30 min of collection.

The samples were placed on ice for approximately 30 min; thereafter, plasma was separated by centrifugation (3000 rpm, 15 min, 4 °C) and frozen at – 80 °C until assayed.