The highest 30-min concentrations were 1,002,456 spores/m indoors and 259,200 spores/m outdoors.
During the first 17 min methanol concentration was increased to 100%% starting at 80%%.
During the first 17 min methanol concentration was increased starting at 80 to 100%.
Following centrifugation (16000 g, 1°C, 5 min), AsAt concentration was determined by HPLC as described above.
After centrifugation at 200× g at 4°C for 5 min, protein concentration was determined with a BCA protein assay kit (Thermo Scientific, purchased via THP, Vienna, Austria).
For preparation of total homogenates, tissues were weighed, homogenized in a 10-fold volume of buffer and centrifuged at 510 × g and 4 °C for 5 min Protein concentration was determined with the Pierce BCA™ Protein Assay Kit Fisher Scientific Austria GmbHH, Vienna, Austria).
After 7.0 min the acetonitrile concentration was decreased to 20% (7.2 min) and was kept constant until end of the run (14.0 min).
Samples were kept on ice for 30 min, with brief vortexing every 5 min and centrifuged at 14,000 rpm for 10 min. Protein concentration was determined using the Bradford Assay (BioRad).
The solubilized lysates were transferred into centrifuge tubes and left at 4°C for 15 min, then pre-cleared by centrifuging at 13,000×g for 15 min. Protein concentration was evaluated with the QuantiPro™ BCA assay kit (Sigma; St .Louis, Mo, USA).
Cells were lysed on ice for 30 min and lysates were clarified by centrifugation at 500 g for 5 min. Protein concentration was determined by Bradford method (Bio-Rad).
