Sentence examples for min for cells from inspiring English sources

For Accutase subculture onto Transwells, treatment length varied depending on differentiation state of the cells (20 25 min for cells without RA treatment, 30 45 min for cells with RA treatment, until a singularized cell suspension was formed).

The acid challenge was maintained for 15 min for cells that were acid sensitive, and 30 min for acid-resistant cells.

In order to obtain a detectable signal of pyruvate in the linear phase of the kinetics, the reaction time was set to 20 min for cells expressing the AGT-Ma/AGT-Ma, AGT-Mi/AGT-Ma and AGT-Mi/AGT-Mi constructs, and to 60 min for cells expressing the other constructs.

Assuming the times of appearance and disappearance of foci mark replication initiation and termination, respectively, then this value for S phase, determined for timelapse cells growing on slides, is slightly longer than our previous estimate of ∼55 min for cells growing in the same liquid medium (Wang et al., 2005).

Cells expressing GstBC134A/C137A from the multicopy plasmid were fully resistant to arsenate and grew in the presence of 1 mM sodium arsenate with a doubling time of 200 ± 10 min compared to 205 ± 10 min for cells expressing wild type GstB.

Overall, we used 25 µm for side length of all microwells, 3.2 × 105 Cells mL−1 for cell density and 3 min for cell trapping time per cycle to realize 85% capture efficiency, which is enough for EGFR mutation analysis.

CSF was obtained by lumbar puncture using a 22-gauge Quincke-type needle, centrifuged (3000 g for 3 min) for cell count, and the supernatant fluid was stored at −80° C until thawed for PC-PLC or total protein assay.

The samples were incubated at 65°C for 10 min for cell lysis.

The plate was incubated at RT for 30 min for cell attachment before being placed at 37°C for 24 h.

Following further incubations at 37°C (30 min for cell homogenates, 15 min for CYP1A1 microsomes), reactions were terminated by addition of 3 ml ice-cold acetonitrile.

The sample was mixed for 2 min for cell lysis, and allowed to stand for 10 min to stabilize the luminescent signal.