Sentence examples for min for cell from inspiring English sources

Slides were washed twice with DEPC-PBS-T, and followed by a post-fixation step in 3.7% (w/v) paraformaldehyde in DEPC-PBS for 30 min (5 min for cell line) at room temperature.

CSF was obtained by lumbar puncture using a 22-gauge Quincke-type needle, centrifuged (3000 g for 3 min) for cell count, and the supernatant fluid was stored at −80° C until thawed for PC-PLC or total protein assay.

Overall, we used 25 µm for side length of all microwells, 3.2 × 105 Cells mL−1 for cell density and 3 min for cell trapping time per cycle to realize 85% capture efficiency, which is enough for EGFR mutation analysis.

The samples were incubated at 65°C for 10 min for cell lysis.

The sample was mixed for 2 min for cell lysis, and allowed to stand for 10 min to stabilize the luminescent signal.

The following day, cells were loaded with 1 μM Hoechst dye for nuclear imaging for 30 min for cell segmentation purposes.

For Accutase subculture onto Transwells, treatment length varied depending on differentiation state of the cells (20 25 min for cells without RA treatment, 30 45 min for cells with RA treatment, until a singularized cell suspension was formed).

The acid challenge was maintained for 15 min for cells that were acid sensitive, and 30 min for acid-resistant cells.

In order to obtain a detectable signal of pyruvate in the linear phase of the kinetics, the reaction time was set to 20 min for cells expressing the AGT-Ma/AGT-Ma, AGT-Mi/AGT-Ma and AGT-Mi/AGT-Mi constructs, and to 60 min for cells expressing the other constructs.

Then, Hela cells were washed with PBS and incubated in a nanogel-free medium and treated with an 808-nm laser at 400 mW/cm2 for 15 min and a 680-nm LED lamp at 10 mW/cm2 for 40 min. For cell survival test, the irradiated plates were returned to the incubator, and cell viability was colorimetrically measured 48 h later with MTT assay [23].

Plates were then blocked with 1% BSA in HBSS for 30 min. For cell adhesion to HUVEC monolayers, 2×105 HUVECs cells were grown to confluence on collagen-coated Transwell membranes, and then treated for 6 h with 25ng/ml TNF-α [51], [52].