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Exact(6)
Cells were washed as described above and PI was added at 5 µl (stock = 200 µg/ml) per milliliter of cells before analysis.
Ten milliliter of cells was harvested by centrifugation at 10,000× g for 5 min at 4 °C.
One milliliter of cells was centrifuged, washed, and resuspended in 10 mmol/L Tris, pH 7.2, 20 mmol/L NaCl, 50 mmol/L EDTA.
One milliliter of cells was washed twice with TLD medium and then resuspended in 0.1 (recB) or 0.5 (parental) ml of TLD medium with 1 µg/ml of DAPI and 1 µg/ml of propidium iodide (PI).
Resuspend B16-F10 cells in pre-warmed PBS at a final concentration of 1 × × 1010 / mL○ cells/ml Add 2 μL of 5 mM stock 5- and -6) carboxyfluorescein diacetate succinimidyl ester (CFSE) (Invitrogen, Carlsbad, CA) solution per milliliter of cells for a final working concentration of 10 μM.
One milliliter of cells was washed twice with M9 with 0.1% glucose and 0.5% casamino acids but lacking thymine (TLD medium), then resuspended in 2 ml of TLD medium at ∼5 × 10 cells/ml and incubated at 37° for 5 hr with aliquots taken and dilutions plated at the indicated times.
Similar(54)
One milliliter of cell-free culture extract was precipitated by adding 250 μL 100 % TCA.
Values are picograms per milliliter of cell culture media.
One milliliter of cell suspension was transferred into 1 L of culture media.
One milliliter of cell culture was pelleted and used to make a cell lysate for Western blotting.
One milliliter of cell lysate was incubated overnight at 4°C with 5 μL of agarose-conjugated anti-p53 antibody.
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