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Values are picograms per milliliter of cell culture media.
One milliliter of cell suspension was transferred into 1 L of culture media.
One milliliter of cell lysate was incubated overnight at 4°C with 5 μL of agarose-conjugated anti-p53 antibody.
One milliliter of cell culture was pelleted and used to make a cell lysate for Western blotting.
One milliliter of cell suspension was added to each sterile 25 mL polycarbonate universal tube and centrifuged at 400G for 5 min at 4°C.
The volume of the cell pellet is measured and, for every milliliter of cell pellet, 10 ml of 30° spheroplasting solution (50 mM KPO4, pH 7.5, 1.0 M Sorbitol and 10 mM DTT) are added.
Similar(51)
For macroscale system, it mainly relies on large laboratory equipment, with the usage of a few milliliters (mL) of cell suspension (e.g., centrifuge).
One milliliter of cell-free culture extract was precipitated by adding 250 μL 100 % TCA.
Cells were washed as described above and PI was added at 5 µl (stock = 200 µg/ml) per milliliter of cells before analysis.
Ten milliliter of cells was harvested by centrifugation at 10,000× g for 5 min at 4 °C.
One milliliter of cells was centrifuged, washed, and resuspended in 10 mmol/L Tris, pH 7.2, 20 mmol/L NaCl, 50 mmol/L EDTA.
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