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To investigate the effect of the expression of the above three c-CBL mutants on cell migration, we carried out wound healing assay as described in materials and methods.
To distinguish between the variants of similar migration, we carried out immunoprecipitations with splice variant-selective antibodies.
To determine the role of SAP in granulocyte migration, we carried out migration assays using a Boyden chamber.
To learn whether the absence of RecA was responsible for the defective swarming migration, we carried out complementation experiments.
In order to avoid mistaking the effects of selection for those of drift or migration, we carried out preliminary tests of selective neutrality.
Similar(55)
In addition, to avoid the off-target effects of Src inhibitors and validate the role of Src on PLLp migration, we also carried out rescue experiments (Fig. 6).
4 We did not adjust for stage at diagnosis owing to the likelihood of stage migration, 23 but we carried out further analyses stratified by stage.
To assess whether PlexinA1 knockdown affects migration in vivo, we carried out in utero electroporation of the MGE of E14 mouse embryos with control-siRNA and PlexinA1-siRNA (n = 4 each group) (Fig. 3D F).
To establish whether the mechanism by which Rho promotes the fascin-1/actin interaction affects the fascin-1/cPKC complex, which is a known negative regulator of actin-bundling by fascin-1, cell protrusions, and cell migration [ 5, 18, 19], we carried out FRET/FLIM measurements for the interaction of GFP-fascin-1 with cPKC-mRFP in control or inhibitor-treated cells.
To identify an upstream target in the control of cancer cell membrane fluidity and migration exerted notably by CAFs, we carried out Western blotting and ELISA and evaluated protein expression and DNA binding activity of the SCD1 transcription factor (SREBP1) in breast cancer cells after 6 days of co-culture with NFs or CAFs.
To confirm this, we carried out transwell migration assays.
Related(20)
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