To investigate the mechanisms regulating this migration, we utilize mAbs selective for conformational epitopes as probes for active LFA-1.
Next, to further explore whether the FSCN1 protein is required for miR-133a-mediated changes in breast cancer cell migration and invasion, we utilized FSCN1 siRNA to knockdown of FSCN1 expression and performed the transwell assays.
In order to identify whether IL-6 was the specific inducer of migration and growth of BMSCs, we utilized IL-6 knockout mice to generate BMSCs and macrophages.
We utilized a tumor spheroid-based migration assay that resembles tumor cell dissemination from a solid microtumor or micrometastasis in terms of engagement of host stromal matrix proteins with characteristics that differ from isolated cells [ 39- 41].
We utilized an established in vitro wound migration assay [ 20].
To determine whether migration of tumor cells across the BBB is dependent on plasmin we utilized an in vitro BBB model using human brain microvascular endothelial cells (BMEC).
For these studies, we utilized chemical inhibitors of select signaling molecules and performed short-term (3 hrs) migration and invasion assays.
To this end, we utilized δD in feathers from hatch-year (HY) lesser scaup harvested during their fall migration throughout the Atlantic, Mississippi, Central, and Pacific Flyways in the United States.
We utilized a micro-wound assay, or scratch test, as described previously [ 47- 49] to assess meniscal cell migration and proliferation in monolayer culture (n = three or more wells per treatment group, each from a different animal).
