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(C ) E19.5 quantification of migration in control and Gsk3 over-expressing neurons using 8 bin analysis as previously described.
Migration in control wells, in which FBS was replaced with 0.1% BSA was minimal (%migration <2.4±1.5%).
The delicate balance between the stimulatory and toxic effects of ROS was also noted when experimentally increasing ROS actually decreased migration in control cells.
Similarly, N-ter and L14-15 significantly reduced migration in control A549, H1299 and H460 cells but had no effect after Mcl-1 knockdown.
10.7554/eLife.09206.023 Appendix figure 7. Robustness of the anterior and posterior fronts dynamics and average filament velocity during the ring migration in control case with J p = − 0. 0..
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As expected in untransfected SH-SY5Y cells, our migration assay using a transwell chamber showed a positive NRG1-stimulated migration in control-vector transfected cells (Figure 8).
Of note, only cells from GCA patients were able to significantly invade Matrigel chambers in the presence of 10% FCS (P = 0.0175, compared to cells from controls) as was also observed for exogenous PDGF (P <0.0001 vs. cell migration in controls).
In STK35L1-silenced cells, we could not observe the stable lamellipodia formation in the direction of migration (like in control), but we found transient lamellipodia formation in all the directions (Movie S2).
(E ) Boxplot comparing the migration speeds in control vs MZ amotl2a −/− mutant pLLP.
Neither CSD nor these reagents significantly inhibited the low level of basal migration observed in control C monocytes not activated with TGFβ.
DNA migration in positive controls had to be > 30%.
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