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In this work, we, by using an E. coli expression system, captured a series of images of NPΔ451 739 conformers at different stages of NLC assembly by negative-stain electron microscopy, which allowed us to picture the dynamic procedure of EBOV nucleocapsid assembly.
Here, we have accomplished this by using TIRF microscopy, which allowed high resolution imaging of the MT cytoskeleton for 1-h time periods, during which Wnt5a promotes cortical axon outgrowth and repulsive growth cone turning (Li et al., 2009).
The lateral resolution of SIM is at least 2-fold greater than confocal microscopy, which allowed us to show that the apparent overlap of VGlut1 and 2 in P15 WT cortex with confocal could essentially be resolved to discrete VGlut1 and VGlut2 puncta with SIM.
Since the data obtained from the skin demonstrated an anti-inflammatory role for sdc-3, we further investigated its role using the more direct approach of intravital microscopy, which allowed real-time dynamic images of leukocyte adhesion to be monitored in anaesthetised mice in vivo.
For this reason, we extended the microscopic documentation of GFP-RabA1d in relation to root hair growth kinetics by means of light-sheet microscopy, which allowed following the distribution of GFP-RabA1d for a considerable time encompassing the entire process of root hair growth, from emergence to cessation of elongation.
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Samples were imaged by Focused Ion Beam (FIB) microscopy which allows high contrast imaging [32].
"At the very microscopic level," he says, "we have developed techniques like two-photon microscopy, which allows extremely detailed examinations of structures and processes within cells".
One of these, routinely used in our work, is atomic force microscopy which allows us to image and manipulate single molecules, cells, and membranes.
This limitation is overcome by microscopy which allows quantitative analysis of biological specimens in time and space and remains the main way to monitor the fate of cells.
We confirmed the occurrence of multivesicular complexes using transmission electron microscopy, which allows more unequivocal identification of the granule membranes.
The cytokinesis-related localization patterns of GFP-RabA1d were further followed by light-sheet microscopy which allows the observation of a physiological, unstressed root growth [ 46].
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