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We combined this motion-cancellation system with differential illumination focal filtering, a variant of HiLo microscopy, which enabled us to image the brain of a freely swimming larval zebrafish for more than an hour.
Excised human skin was investigated by means of fluorescence microscopy, which enabled the detection of significant increment in the penetration of tNG as well as the encapsulated fluorescein.
The thermal imaging of an active nucleation site was performed by utilizing high-resolution, fluorescence microscopy, which enabled a maximum spatial resolution of 370 nm/pixel.
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This feature is somewhat similar to digital holographic microscopy, which enables quantitative 3D phase imaging of unlabeled live cells48,49.
Membrane curvature detection with PLM requires fewer localization events to detect curvature than three-dimensional single-molecule localization microscopy (e.g., photoactivated localization microscopy or stochastic optical reconstruction microscopy), which enables curvature detection 10× faster via PLM.
To control the amplitude and frequency of Msn2 activation, they combined high-throughput microfluidics, which enables the rapid delivery and removal of an Msn2-activiting small molecule, with quantitative time-lapse microscopy, which enables the measurement of fluorescent gene expression output.
Fluorescence microscopy, which enables the observation of living cell structures, organelles and even small molecules, plays an indispensable role in life science.
Thus, we assessed the effects of AGEs on actin cytoskeletal redistribution using fluorescence confocal microscopy, which enables visualisation of profilin-1 and actin filaments.
Total internal reflection fluorescence (TIRF) microscopy, which enables high-resolution assessment of molecules close to the plasma membrane, suggested that mATG9 redistributed close to the plasma membrane in Dynasore-treated cells.
Another 3D imaging modality at higher resolutions for in vivo applications is confocal microscopy which enables the visualization of superficial layers at a depth of up to a few hundred micrometers with submicrometer resolution.
Particularly suited for cancer research is infrared multiphoton microscopy, which enables deep tissue penetration and detects multicellular, collective invasion of melanoma and soft-tissue sarcoma lesions in vivo [8].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com