Under fluorescence microscopy, there were several GFP+ Sk-34-derived cells in the DsRED mouse cardiomyocyte spheres after 6 days of culture (Fig. 1B).
Between microscopy and assisted virtual microscopy there were 87 matches (72.5%) and 33 mismatches (27.5%).
On light microscopy, there were 31 45 glomeruli per level section, of which 1 2 were globally sclerosed.
On scanning electron microscopy, there were budding polarity and ruffling changes in every field, but there were no consistent differences (data not shown).
In the clinics in the north with new microscopy, there were 39/193 (20%) false positive results, giving a specificity of 79.8% (table 5).
In the clinics in the east with established microscopy, there were 329/1212 (27.1%) false positive results (χ=2.8, P=0.1).
When aorta, coronary, renal, hepatic, and splenic arteries in BM-transplanted eNOS−/− mice were examined by confocal microscopy, there were no EGFP- or eNOS-positive endothelial cells detected in these vessels in any of the treatment groups.
By visible light microscopy, there were approximately 25% dead cells (stained with trypan blue) and 30% apoptotic cells (unstained with trypan blue and abnormal morphologically, many having a condensed, fragmented nucleus).
