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We also implemented for the first time lattice light-sheet microscopy in zebrafish larvae.

We then investigated the functional capability of this construct and response to a range of potent and weak environmental oestrogens using a transient expression assay using green fluorescent microscopy in zebrafish embryos/larvae.

Analysis of time-lapse microscopy in zebrafish reveals that atorvastatin, in 90% of cases, induces vessel rupture and bleedings in the region of the central arteries at the time when these vessels start to sprout from the primordial hindbrain channel.

Implications and future directions These findings show that intravital microscopy in zebrafish can be used to discover immune maintenance mechanisms in the brain that would be missed using other approaches.

Accordingly, we have established an experimental paradigm that combines traumatic neural injury and multiparametric intravital microscopy in zebrafish to study in detail the behavior and function of Schwann cells during the repair of a sensory circuit.

To identify the cellular origin of this massive increase, we performed in vivo time-lapse microscopy in zebrafish at the corresponding stages, starting at 16.5 hpf, in a transgenic line expressing a membrane-coupled GFP in retinal stem and progenitor cells (Rx2 GFPcaax).

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Using time-lapse confocal microscopy to observe nuclear movements in zebrafish retinal neuroepithelial cells, we show that, except for brief apical nuclear translocations preceding mitosis, IKNM is stochastic rather than smooth and directed.

To this end, the spatiotemporal activity of hoxb4a was documented from 1 30 days in specific subgroups of r7 8 neurons using high-resolution confocal microscopy in an enhancer-trap zebrafish line with yellow fluorescent protein (YFP) retroviral insertion [27], [28] that closely reproduces the endogenous mRNA expression.

With least scattering and absorption attenuation, THG microscopy in thick (~1 mm) zebrafish embryo [ 15] or two-photon fluorescence imaging in deep (~1.6 mm) mouse cortex [ 21] have been demonstrated in vivo.

In this study, we implemented another method by using a high-resolution PAM method, optical-resolution photoacoustic microscopy (OR-PAM), to image microstructures in zebrafish larvae.

Discontinuous measurements of cellular velocities have been made in zebrafish embryos using video microscopy, and in transgenic mouse embryos expressing GFP-labeled erythrocytes using perpendicular linescans [ 15, 19].

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