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Modern microscopy techniques, including fluorescence and electron microscopy, have allowed us to gain insights into the molecular organization of cells.
Different techniques, particularly, high-resolution transmission electron microscopy, have allowed to determine the microstructure of these nanofilaments.
Furthermore, high penetration depth and three-dimensional sectioning capability intrinsic to CARS microscopy have allowed its application to tissue and live animal imaging [13].
Advances in sample preparation and electron microscopy have allowed the structure of cilia to be explored at an unprecedented level of detail.
Recent advances in fluorophore labelling of plant cell walls using a modified pseudo-Schiff-propidium iodide (mPS-PI) staining procedure in combination with high-resolution confocal microscopy have allowed visualization of cellular details of individual tissue layers in whole mounts, hence enabling study of tissue and cellular architecture without the need for tissue sectioning.
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Confocal microscopy has allowed optical sectioning and reconstruction of tissues in three dimensions.
Transmission electron microscopy has allowed the identification of ultrastructural features of the adult S. japonicum, some of which differ from the reported features of other schistosome species.
Up to now, fluorescence microscopy has allowed us to probe the larger scale organization of chromosome territories in the micron length scales, however, the smaller length scales remained invisible due to the diffraction limited spatial resolution of fluorescence microscopy.
The advent of single molecule fluorescence microscopy has allowed experimental molecular biophysics and biochemistry to transcend traditional ensemble measurements, where the behavior of individual proteins could not be precisely sampled.
More recently, electron microscopy has allowed researchers to study behavioral patterns, and DNA amplification has helped with genetic tests that determine the phylogenetic relationships between the extinct and living lemurs.
Moreover, laser-scanning CARS microscopy has allowed label-free monitoring of lysophosphatidylcholine-induced myelin degradation [18].
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