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Objects as small as 0.02 nm are resolvable by electron microscopy, compared with 0.25 μm allowing, for instance, the observation of virions and viral structures.
Reduced specificity of concentrated microscopy compared with direct smears has been reported previously [ 10, 11].
The observed low level of sensitivity of microscopy compared with that of PCR for identifying P. falciparum infections is similar to findings observed elsewhere (12 – 12 ).
Associated HIV infection was believed to account for low specificity of LED-FM microscopy compared with the conventional ZN microscopy in two previous studies in settings with high burden of the disease 29 30 As expected, the digestion and decontamination with NALC–sodium hydroxide (NaOH) produces the highest yield of AFB in the sputum exposed to the reagent for 15 min.
We evaluated the feasibility of an early prototype ligand-coated magnetic bead technology to concentrate M. tuberculosis prior to detection by LED-based fluorescence microscopy compared with direct Ziehl-Neelsen microscopy and direct and concentrated fluorescence microscopy in a reference laboratory in Kampala, Uganda.
Similar(55)
The measurements of the geometry of periodic structures obtained using acoustic microscopy are compared with those obtained using optical microscopy, and the reliability of this acoustic technique is also examined.
This result supports a study by Krippendorf and Lough [ 20], which demonstrated a significant improvement in the identification of tissue structure and case analysis exam performance by students who learned using virtual microscopy when compared with those who used conventional microscopy.
Their response was monitored via microscopy, including both fluorescent and video microscopy, and compared with that seen by cell lines of monocytes/macrophages (THP-1 and J774 cells), considered to be committed phagocytes, in addition to a nonmacrophage cell line (L929 fibroblasts).
Overall, 21% (32/154) of patients with a negative clinic slide result were treated with a malaria drug in the new microscopy arm compared with 270/905 (28.8%) in the established microscopy arm (χ=5.3, P=0.02).
Nebulin fragment incorporation was monitored by fluorescent microscopy and compared with the distribution of actin, α-actinin and titin.
Cells challenged with 75 mM of Cs+ or Sr2+ for 24 h were subjected to field emission scanning electron microscopy and compared with the control, metal unchallenged cells.
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Justyna Jupowicz-Kozak
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