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Visible spheres were counted under a microscope on day 8 after plating.
Brain tissue sections from each group were H&E stained and examined under the light microscope on day 5.
Nonadherent cells were removed after 3 days of culture, and colony forming units (CFU) were counted under light microscope on day 7 (6 wells per measuring point).
The colonies were counted using an inverted microscope on day 11 for BFU-E and on day 14 for CFU-GM.
Colony formation of cells was monitored for 7 days in 37°C incubator and colony number and size was recorded for comparison, and microphotographed by Axiovert 40 CFL microscope on day 7.
Briefly, all eyes were examined by a blinded ophthalmologist under slit lamp microscope on day 1, 2, 5 and 8 after alkali burn.
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His bowling will be under the microscope on Friday, though, with the Australians picking three specialist bowlers.
Wayne Rooney is under the World Cup microscope and on Thursday the eyes of a nation - if not the world - will be on the England striker.
Stained slides were imaged using Talamasca 2P confocal microscope on the same day.
Embryo quality was evaluated under inverted microscope on the second day of development according to Veeck [ 24].
EGFP+ fish were screened under the fluorescent microscope on the next day and bred up to sexual maturity, then crossed with the WT AB fish.
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