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Frequency modulation of an atomic force microscope is based on a PI control law, which keeps the amplitude equal to a desired value.

The microscope is based on a TE200 body with a ×4 (NA 0.16) air objective lens (Nikon, UK) and comprises a 3-CCD colour camera (KY-F75U, JVC UK), motorised stage (Scan IM 120 × 100, Marzhauser, Germany) and in-house written software [25]).

Our reflection mode lensfree microscope is based on off-axis digital holography.

The microscope is based on light interferometry and operates as a noncontact optical profiler in vertical scanning interferometry mode to produce 3D topography maps of the sample surface.

The nonlinear spectral imaging microscope is based on a homemade inverted microscope system described in previous work [ 20] and diagram is shown in Fig. 1.

As described in Methods, our microscope is based on circularly polarized light, allowing contrast to be independent of crystal orientation within the plane perpendicular to the microscope's optical axis, and generating images in which intensity is proportional to birefringent retardance.

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The calibration process for the microscope, which is based on image-charge electrostatic models, has been adapted to use the Laplacian 'complex frequency'.

The confocal laser scanning microscope used in this study is based on the Heidelberg Retina Tomograph II scanning laser system in combination with the Rostock Cornea Module (image modality 1) or a rigid endoscope (image modality 2).

The interference microscope used for this work is based upon a Linnik configuration (Fig. 1 ) [ 5, 15].

A new hand-held intravital microscope has been developed recently that is based on incident dark-field imaging [ 22], containing a computer-controlled high-resolution imaging sensor [ 61].

This system is based on an upright microscope with attached spectrometer.

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