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Each culture was gram stained (Fisher Scientific) and checked for pure culture conditions under the microscope for each experiment.
In these experiments, cell occupancy was measured by counting the cell number in 60 randomly chosen droplets using a fluorescent microscope for each tested cell concentration.
At least 400 cells were scored under fluorescence microscope for each experiment.
Different fields of view (>3 regions) were analyzed on the microscope for each labeling condition, and representative results are shown.
The percentages of differentiated cells were calculated based on a total of 200 cells counted randomly for approximately 5 different fields under microscope for each experiment.
With the digital confocal microscope, for each scanning position, we can capture the entire optical field falling within the numerical aperture of the imaging objective.
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He arrived at this number by first measuring the animal using eye-micrometers in his microscope, for which each division was 1/53 mm and 1/28 mm.
Randomly chosen fields (n = 20) were photographed with a Zeiss bright-field microscope and for each microscopic field, the area stained with DAB (the HEV area) and the total area of the gland was measured with NIH-Image software (5 fields/mouse).
In the present study, we present a hierarchical model based on faecal egg counts (FECs; expressed in eggs per 1 g of stool) in which we first describe the variation in FECs between individuals in a particular population, followed by describing the variance due to counting eggs under a microscope separately for each stool sample.
Seven micron frozen sections were cut and attached to uncharged microscope slides for each sample.
Two images of each corallite were taken in separate sessions (i.e. the colony was positioned under the microscope separately for each image).
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