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Once the fluidics steps were executed, the post-fixation/staining imaging was then automatically performed taking a 3D z-stack of 30 40 planes (spaced by 0.5 μm) in DAPI, GFP, mCherry and AlexaFluor647 channels (200 ms exposure times and 20% illumination for each channel).
Recessed directional lights provide focused illumination for each piece of art — or, in this case, for an alcove dedicated to a sculptural bouquet of flowers.
We used curves fitted to our acuity-illumination (Fig. 1), acuity-contrast (Fig. 2), and acuity-viewing distance (Fig. 3) functions to describe a series of "threshold acuity surfaces" which relate acuity to target contrast and illumination, for each viewing distance (Fig. 4).
Images were corrected for uneven illumination for each channel (DAPI and Alexa488 signals) and converted into binary images by grayscale Image Thresholding.
Analysis of joint section (synovial membrane and cartilage) was performed by using an Axioskop 2 phase-contrast/epifluorescence microscope (Carl Zeiss, Inc., Toronto, ON, Canada) equipped with a 10× or 40× objective at the same level of incident illumination for each slide in the experiment.
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The illumination spectra for each pixel (which include the influence of both the illuminant and scene geometry) were then determined by dividing the radiance spectrum by reflectance spectrum.
A considerable degree of care is taken to discuss the types of prints referenced and the color temperature of the viewing illumination used for each print.
This result can be used to estimate the pose and structure of the face and the illumination conditions for each frame in a video sequence in the presence of multiple point and extended light sources.
The mean value and standard deviation were calculated using the data obtained for three 24 h illumination cycles for each individual culture.
An optical diffuser (made using three sheets of A4 printer paper) was inserted between the LEDs and the cuvettes for uniform illumination of each cuvette.
History is there to be mined or undermined, renewed or debunked, as each generation ferrets out illumination for their times.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com