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Exact(5)
Confocal microscopy was performed with a Nikon or Zeiss confocal microscope as indicated.
Four areas around the femoral vein were determined at ×1 magnification under microscope as indicated in Figure 1.
Lung lesions were visualized under dissecting microscope as indicated by arrows and by H&E staining and histology showing clear metastasis (indicated by arrows).
Rinse pegs in 0.9% saline for 1 min. Stain pegs with the appropriate fluorphores and examine using the confocal laser scanning microscope as indicated in Protocol 6. Break pegs from the CBD using a pair of flamed pliers.
Rinse pegs in 0.9% saline for 1 min. Stain pegs with the appropriate fluorphores and examine using the confocal laser scanning microscope as indicated in Protocol 6. 0.9% saline 96-well microtiter plate Micropipette tips 2-2000 μL), in racks of 960.9%% saline 96-well microtiter plate Micropipette tips 2-2000 μL), in racks of 96 **There is an option here to fix the biofilms using glutaraldehyde.
Similar(55)
Cells were imaged using the Olympus Fluoview FV-1000 Laser Confocal Microscope (Toronto, ON, Canada) and software with × 20, × 40, or × 60 oil-immersion objectives, as indicated.
Statistical significance was assessed as indicated.
Test, publish, review and change as indicated.
The medium was immediately replaced, and spontaneous cell migration was monitored using a Nikon inverted microscope for 18 24 hours, as indicated.
Immunofluorescence was viewed using a Nikon 90i inverted phase contrast microscope using 20× and 40× objectives, as indicated, and analyzed using NIS Elements Advanced Research software.
The cells were then observed under a microscope for the cytopathic effect (CPE) of the virus as indicated by disruption of cell monolayer and change of shape from spindle to round.
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